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General Category => Yeast and Fermentation => Topic started by: 1vertical on October 31, 2010, 01:24:01 AM

Title: Growing up an alien wild yeast from scratch
Post by: 1vertical on October 31, 2010, 01:24:01 AM
anybody done this or have a process that works well??
I have a culture that I want to try to build up to beer fermentation levels
It is on the stir plate and I have been slowly trying to build the numbers of
yeasties by doubling the pitch rates over and over...cept I got side lined and
forgot to proceed and the yeast sat a long time in the fridge....I am trying to
give it a start again..we will see...I have minimal activity in a quart of starter wert.
Title: Re: Growing up an alien wild yeast from scratch
Post by: pyrite on October 31, 2010, 01:49:12 AM
Alien?
Do you know what type of wild yeast it is?
What's it smell like?
Title: Re: Growing up an alien wild yeast from scratch
Post by: hopfenundmalz on October 31, 2010, 01:52:29 AM
Do you live near Roswell?   ;D
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on October 31, 2010, 01:56:17 AM
Do you live near Roswell?   ;D

1K miles from roswell...ha ha ha
No this was a yeast that started in a bottle of V8 Peach Mango Fusion in the
fridge at 40 something degrees....but Now it is on the stirplate and is demonstrating
top fermentation characteristerics...little round colonies...I pitched it into a quart starter
and prolly did not have many viable cells to begin with.  

Alien because I do NOT know what it is...ale or lager  or Brett...I drank some of the last
starter that was with this yeast that had completed. It tastes like chanterelle mushrooms smell
kind of fruity...like apricot but different.  Maybe it is a Brett I do not know, but I am gonna brew
with it if I can get it going...
It is really pleasant
Title: Re: Growing up an alien wild yeast from scratch
Post by: pyrite on October 31, 2010, 02:09:47 AM
wish I could help, but I've only fiddled with the brett series and the sour mixes.  I'm curious to find out how your wild stuff turns out.
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on October 31, 2010, 05:35:40 AM
Feed it slowly with low gravity wort, use some yeast nutrient, and be patient.  If it's going to have to sit again, once it settles dump off the media and add some distilled water.  It will stay happier longer.

But what do you mean by "little round colonies" when it is on a stirplate?   :-\
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on October 31, 2010, 05:47:54 AM
Tom,
I ran the stir bar for 24 hours and saw nothing...so I figured the wort was full of O2...
So I turned it off to see if I could detect anything....then you know how when a carboy is done
with primary and sometimes those little round colonies of yeasties are on the surface???
Well that is what this looked like only in a quart size carboy...lol... I now am seeing a good
amount of foam covering the surface and think that the thing is gonna be good to go....I will
try and get a photo...

Edit: looking fairly normal now with activity beginning. Just got off to a slow start methinks its gonna be alright
(http://i9.photobucket.com/albums/a87/Vertical1/WildStirplate.jpg)
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on October 31, 2010, 06:25:55 AM
Tom,
I ran the stir bar for 24 hours and saw nothing...so I figured the wort was full of O2...
So I turned it off to see if I could detect anything....then you know how when a carboy is done
with primary and sometimes those little round colonies of yeasties are on the surface???
Cool, I get it.  I don't think of that as a colony, but that doesn't mean you can't call it that. To me a colony grows from a single cell on a plate, so I was just confused.  No worries.  :)

You are most likely culturing a mixed culture.  Without isolating colonies, it will be hard to know what it is exactly.  If you look at it under a microscope you might be able to spot the yeast and bacteria that are likely present, if only to confirm that there is a mix.  Even if you see only yeast it is likely a mix of yeast.

If you isolate colonies, you can inoculate them in media containing cycloheximide at 5-10 ug/ml.  If it grows, it is probably Brett.  If it doesn't grow, it is probably Sacc.  If you inoculate a mixed culture and get growth then there is probably some Brett, but maybe not only Brett, but if it doesn't grow it is probably only Sacc.

Taste it as you go, because since it is likely a mixed culture it is probable that one strain will dominate at different points and eventually it might not be to your liking.

HTH

<edit> fixed typo
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on October 31, 2010, 06:37:44 AM
My microscope is a stereo 40X at best. i use it for mineral examining. Not enuff umph for yeast and bacteria.
As for the cyclohexamide, i have none laying around and no avenue to obtain any....this is part of the process
that will best be served by someone else.  I will if this is decent, part with a quantity for such purposes.

That flavor was simply amazing and good...hope it stays with the organisms.
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on October 31, 2010, 07:04:34 AM
If it turns out well and you like it and want to know more, send me a sample.  I'll see what I can do.
Title: Re: Growing up an alien wild yeast from scratch
Post by: bluesman on October 31, 2010, 03:59:21 PM
This is interesting. Again...how did you get the wild yeast? It was spontaneously started in a V8 product?
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on October 31, 2010, 04:29:26 PM
This is interesting. Again...how did you get the wild yeast? It was spontaneously started in a V8 product?


Yep, I had a jug of V8 Peach Mango Fusion...not to be confused with the other V8 product with a similar
name that reverses the order of the fruit in the title....It was open in the fridge for a LONG time and I went
to get a glass noticed the jug was rock hard...thought....hmmmm....cracked it open PSSSSSSSST...

CO2 exited the container ....thought....hmmmmmm.....poured into a glass....fizzy bubbly ...
thought hmmmmmm.....tasted.....thought WOW GREAT flavor.

So good in fact, now I carb up 2 liter bottles with my carbonator just to have carbed V8 peach mango fusion
over a bit of ice.... ;D
Title: Re: Growing up an alien wild yeast from scratch
Post by: bluesman on November 01, 2010, 02:05:27 AM
This is interesting. Again...how did you get the wild yeast? It was spontaneously started in a V8 product?


Yep, I had a jug of V8 Peach Mango Fusion...not to be confused with the other V8 product with a similar
name that reverses the order of the fruit in the title....It was open in the fridge for a LONG time and I went
to get a glass noticed the jug was rock hard...thought....hmmmm....cracked it open PSSSSSSSST...

CO2 exited the container ....thought....hmmmmmm.....poured into a glass....fizzy bubbly ...
thought hmmmmmm.....tasted.....thought WOW GREAT flavor.

So good in fact, now I carb up 2 liter bottles with my carbonator just to have carbed V8 peach mango fusion
over a bit of ice.... ;D

That's groovy man.  ;D

Never would have thought of that but hey...you might be on to something.
I'll be curious to know your findings.
Keep us posted.  ;)
Title: Re: Growing up an alien wild yeast from scratch
Post by: majorvices on November 01, 2010, 12:06:58 PM

You are most likely culturing a mixed culture.


Exactly, so don't be surprised when it changes drastically over the next generation or so, even so much as to change and sour while the finished beer is conditioning. The character you are probably picking up is probably from a wild yeast strain which can sometimes be surprisingly clean. But wild strains never travel alone so all other type of beer souring critters will be along for the ride. I have tried to culture wild strains in the past and have come up with some initially interesting results that all failed during the conditioning period. Usually ended up with a "vomit" flavor in the final beer.
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 01, 2010, 02:31:42 PM
All this tends to make me want to do a 3 gallon trial beer for the 1st go round....
This starter has that mango/apricot/chanterelle mushroom aroma. I think the behavior
is very Brett like.  I should make an apricot beer for this methinks.  That way vomit
flavors will have texture too!  ;D

Update; Today Kreusen has dropped on the starter
Edit: this is the third iteration
Title: Re: Growing up an alien wild yeast from scratch
Post by: ryang on November 01, 2010, 03:53:09 PM
lurking and watching from the stands as a very curious and excited spectator...  (here and BBB)
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 01, 2010, 04:19:57 PM
Ok all you followers of alien wild yeasties...updated photo. I turned off the stir plate and
now you can see those little round colonies...kind of...thru the condensate.  The chunks
around the outside periphy are pieces of hop cones. The yeast/brett is settling out and
when It does, I will try to remember post taste notes...
(http://i9.photobucket.com/albums/a87/Vertical1/colonies.jpg)
Title: Re: Growing up an alien wild yeast from scratch
Post by: bluesman on November 01, 2010, 04:22:45 PM
All this tends to make me want to do a 3 gallon trial beer for the 1st go round....

...or even a one gallon.  :-\
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 01, 2010, 04:51:16 PM
All this tends to make me want to do a 3 gallon trial beer for the 1st go round....

...or even a one gallon.  :-\
This becomes my chance to do a brew in a bag...thing....ahhhhh....the hobby kicks in.....
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 04, 2010, 05:13:32 PM
Update;
Starter activity has ceased and I tasted the liquid despite my apprehension.
Oxygen for sure...dang it...But beyond that,
It is fruity ....some unknown...apricot ...pluot...plum...mangoish.

To me nailing it down is this, It tastes like chanterelle mushrooms smell....  

and that is NOT a bad thing.
I put those other fruit words up there for those of you that have not had the chanterelle experience.
Finish is light and as if It has some carbonation...tho it does NOT.

Next iteration is a 3 gallon beer I hope.
Title: Re: Growing up an alien wild yeast from scratch
Post by: ryang on November 04, 2010, 06:08:32 PM
very cool!
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 05, 2010, 06:26:44 AM
How much yeast sediment are you seeing at the bottom of the container?
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 05, 2010, 02:21:59 PM
How much yeast sediment are you seeing at the bottom of the container?
one thousand words on the question Tom
(http://i9.photobucket.com/albums/a87/Vertical1/Sediment.jpg) ;D

Seems like I have this  much when I grow up a smack pack....BTW, that is a 1/2 gallon Jar.
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 05, 2010, 04:01:18 PM
That looks great.  I can't wait to hear how the batch turns out.
Title: Re: Growing up an alien wild yeast from scratch
Post by: morticaixavier on November 05, 2010, 09:47:21 PM
That looks great.  I can't wait to hear how the batch turns out.

me either! that is so cool. I have been wanting to capture some wild yeasts for a while now. I live just over the way from Somona county where Russian River makes some awefully tasty wild brews and am curious about my local flora (fauna? is there an 'F' word for micro-organisms?)
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 05, 2010, 10:05:04 PM
That looks great.  I can't wait to hear how the batch turns out.

me either! that is so cool. I have been wanting to capture some wild yeasts for a while now. I live just over the way from Somona county where Russian River makes some awefully tasty wild brews and am curious about my local flora (fauna? is there an 'F' word for micro-organisms?)
The word for microorganisms is an 'm' word: microorganisms :)

You can also use microflora, microfauna, microbiota etc.  But someone could come up with pedantic reasons why those words aren't exactly accurate.
Title: Re: Growing up an alien wild yeast from scratch
Post by: morticaixavier on November 05, 2010, 10:50:37 PM
The word for microorganisms is an 'm' word: microorganisms :)

You can also use microflora, microfauna, microbiota etc.  But someone could come up with pedantic reasons why those words aren't exactly accurate.

damn pedants. They're not plants and they're not animals we need a new 'F' word!
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 05, 2010, 11:04:58 PM
forganisms?
Title: Re: Growing up an alien wild yeast from scratch
Post by: bluesman on November 05, 2010, 11:07:49 PM
forganisms?

Say what?  :D
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 05, 2010, 11:10:24 PM
forganisms?

Say what?  :D
Well, it's an f-word for organisms . . .  ;D
Title: Re: Growing up an alien wild yeast from scratch
Post by: bluesman on November 05, 2010, 11:14:47 PM
forganisms?

Say what?  :D
Well, it's an f-word for organisms . . .  ;D

Just yankin' your chain.  ;D
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 06, 2010, 06:00:32 AM
forganisms?

Say what?  :D
Well, it's an f-word for organisms . . .  ;D

Just yankin' your chain.  ;D
;D  Yeah, I have a tendency to yank back.  It's just my sense of humor.   ;)
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 07, 2010, 02:11:26 AM
Base Wert for a Wild Apricot Chantrelle Beer....Hope I do not have too much of the vomit notes, this
is gonna be the beer I pitch that wild yeast into tomorrow after my fridge drops the temp on down
to the 40"s...because this stuff spontaneously took off in the 38-40 deg f range.  I am gonna try to drop
it down and see if it will start cold...
(http://i9.photobucket.com/albums/a87/Vertical1/WildApricotBase.jpg)
Cooling in the refer waiting for oxygen and pitching tomorrow am....

Edit: Pitched @ 47-48 degrees and keep rousing it...wants to take off...but not yet...we will check
in the morning
Edit#2; This morning, albeit slow, methinks it is fermenting. Bubbles are pootin out of the airlock ;D
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 17, 2010, 07:30:26 PM
Update
Today it appears to have completed it's mission and has pooped out...so attenuation
was not that wonderful but then I gave it a high gravity start and a lot of work to do.

We came down to 1.023 today and correcting for temps approx 4.6 % ABV.
Taste is mild and fruity with a hint of peach mango which is not suprising considering the
source.  Smooth and not harsh kind of sweet because of the ending gravity point more time
may change this I just do not know. Taste is ...beer... I get no vomit notes.

An interesting Run for sure....

Pic.... ;D
(http://i9.photobucket.com/albums/a87/Vertical1/AlienWildFG.jpg)
I believe that this will be good to add the apricot extract to the mix and then carb it up for enjoying.
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 17, 2010, 07:41:49 PM
Are you planning to warm it up a bit and see if the gravity drops further?  Or maybe a different yeast?  As long as you're kegging, no problems, but I'd worry about bottling, especially since it ferments at such cold temperatures.  If there's residual sugar then it might keep working slowly in the bottles and building up pressure.
Title: Re: Growing up an alien wild yeast from scratch
Post by: bluesman on November 17, 2010, 07:47:38 PM
+1

warm it up and add some more yeast to help it finish...

Then there's always the option of blending it with some other beer.  8)
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 18, 2010, 05:43:49 AM
I did warm it up about 4 days ago...great minds think alike...no hurries
I caught a couple of good forceful exhales thru the bubbler this evening.
Will check Sp. Gr. again soon...
Title: Re: Growing up an alien wild yeast from scratch
Post by: stlaleman on November 18, 2010, 01:10:14 PM
If it knocks down the krausen, why does it not effect the head on the finished beer?
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 18, 2010, 02:36:56 PM
If it knocks down the krausen, why does it not effect the head on the finished beer?
I will venture a guess and try to answer this, but your post seems off topic.
Krausen is a function of the yeast as they consume their food source.
Dramatic temperature changes can make yeast activity subside...or accelerate depending.

Head is more from the ingredients in the wort like carafoam and wheat...proteins.
And CO2 nucleation points that allow the gas to collect and rise.
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 18, 2010, 05:06:30 PM
If it knocks down the krausen, why does it not effect the head on the finished beer?
I will venture a guess and try to answer this, but your post seems off topic.
Krausen is a function of the yeast as they consume their food source.
Dramatic temperature changes can make yeast activity subside...or accelerate depending.

Head is more from the ingredients in the wort like carafoam and wheat...proteins.
And CO2 nucleation points that allow the gas to collect and rise.
I think this was meant for the fermcap discussion . . . or maybe not, but it makes more sense there :)
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 23, 2010, 06:16:46 AM
Ok help with this I think I want to bottle the 16 bottles that I will get
of this beer because frankly, I am gonna need my kegs for lagers this winter.
My bottles are grolsch swing tops...so I can release pressure after time goes by
should the need arise.

So how do I proceed???, try a packet of us-05 and see if It takes off again?

just bottle it where it is and monitor it closely?

Bottle as usual adding corn sugar for carbonation to the beer?
(I desire to add apricot extract to this to finish it out as well)

As sweet as it is, It needs some help finishing methinks. But
The apricot extract may fool the taste buds a bit.

I see no further evidence of fermentation in primary.
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 23, 2010, 06:54:11 AM
I'd be tempted to keg and force carbonate, then counter-pressure fill and keep the beers cold.  I think it will mitigate a lot of potential problems.
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 23, 2010, 02:12:34 PM
I'd be tempted to keg and force carbonate, then counter-pressure fill and keep the beers cold.  I think it will mitigate a lot of potential problems.
Thanks for that advice Tom, I think that sounds like a good plan.
Title: Re: Growing up an alien wild yeast from scratch
Post by: bluesman on November 23, 2010, 02:35:58 PM
I'd be tempted to keg and force carbonate, then counter-pressure fill and keep the beers cold.  I think it will mitigate a lot of potential problems.

+1

Otherwise...I see bottlebombs in your future.

Good Luck!
Title: Re: Growing up an alien wild yeast from scratch
Post by: ryang on November 23, 2010, 02:57:54 PM
yeah, that would be quite handy.  in my tepache thread, I bottled it with some cane sugar, and I have them all in the sink... I don't trust them at all.  one especially, it's going nuts in the bottle and is actually releasing pressure out of the crown cap... :o
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on November 24, 2010, 08:35:55 AM
yeah, that would be quite handy.  in my tepache thread, I bottled it with some cane sugar, and I have them all in the sink... I don't trust them at all.  one especially, it's going nuts in the bottle and is actually releasing pressure out of the crown cap... :o
Sounds like it's time to chill and drink that one ;)
Title: Re: Growing up an alien wild yeast from scratch
Post by: brewmasternpb on November 26, 2010, 05:45:44 AM
I love your experiment!  It's a cool attitude that you have toward brewing.  My 2 favorite parts of the whole scenario were:
1) Something spontaneously fermented in your fridge, and your first instinct was to drink it  ;D  Only a homebrewer...
2)  I like how the "vomit" character of your wild yeast only seemd like a minor setback... which paid off 
Awesome job!
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on November 26, 2010, 06:17:59 AM
Ok, Thanks for that brewmasternpb.  BTW, that vomit character was a possible and never as of yet manifested in this strain      It is now however in secondary and that is where Keith said these instances occured for him.
 I forgot to update. I elected to try Tom's advice and It is now kegged,
in the stainless, under 25 psi of CO2 carbing up...I added 1.7 ish ounces of Apricot Extract to the beer....
the recommended quantity for 2 gallons...beyond that, I do not know....we wait.... ;)
Title: Re: Growing up an alien wild yeast from scratch
Post by: ryang on January 06, 2011, 03:55:34 PM
Any updates here? 

Tasting notes?
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on January 06, 2011, 04:54:02 PM
ryang, there is more enroute, just needs a bit more time...I can tell you this

The beer SMELLS incredible fruity and like apricot, and then the flavor is well, beer.

So you hoist this up to your nose, and it smells so good and then you get a taste,
and you are expecting the aroma to carry over and it does not...it is just good smelling beer....
not bad tasting, but not anything like you'd expect. 

Tom has plated a sample and needs more time to know what grows on the media.
I am sure he will update us all with that information when it is obtained.
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on January 06, 2011, 10:11:00 PM
I told Dave, I looked at it under a microscope and it appears to be predominantly yeast.  Right size and morphology, some were budding.  There was some debris that made it hard to tell what else might be in there, and anyway, a little bit of bacteria can have a large impact on the beer, which can make them hard to find under a microscope if they're not isolated.

So I've plated it to some yeast growth media, but bacteria might grow on it depending on the strain.  I'm growing it kind of cool, around 20C instead of 30C.  It was growing the fridge though, so that should be fine.  I also plated it to one with an antibiotic that will kill any bacteria but not affect the yeast, so I'll have a purified strain(s?) to play with.

I still need to grab some LB plates to plate it to, I only had ones with antibiotic available.  That will favor bacteria, since there is no sugar for the yeast to grow.
Title: Re: Growing up an alien wild yeast from scratch
Post by: gmac on January 06, 2011, 11:07:32 PM
So if it's a new strain, do you get to name it?  B. V8ensis?

I have a particular F word I use when I find micro-organisms in my beer.  Not really appropriate for the forum.
Great posts.

Would it be worthwhile to make a completely neutral wort to see what base flavour characteristics this would impart?  Or maybe you covered that in the starter when you tasted it ( your mushroom flavours).

Anyway, fantastic idea.  You created a new beer style and discovered carbonated V8.  Just don't tell the V8 people or you'll end up paying a royalty or something.
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on January 06, 2011, 11:35:48 PM
I got an LB plate with no antibiotic, so I'll plate the solution and see what grows.  Unfortunately I couldn't find any with an antibiotic that will kill yeast and not bacteria.  Still, the lack of sugar in the LB plate should limit yeast growth.
Title: Re: Growing up an alien wild yeast from scratch
Post by: oscarvan on January 07, 2011, 12:57:20 AM
Very cool thread. Meanwhile, how about "foama" ?
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on January 07, 2011, 05:21:30 AM
Very cool thread. Meanwhile, how about "foama" ?
???
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on January 07, 2011, 05:44:04 AM
me< Leaning more toward forganism... :D :D :D

Edit:

oscarvan, I get it now;
F =  Forganism
O = Orange (it's color)
A=  Apricot
M=  Mild
A=  Ale

I'll try to put up another photo of this beer. 
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on January 15, 2011, 11:38:35 PM
I'll try to put up another photo of this beer. 

As promised
(http://i9.photobucket.com/albums/a87/Vertical1/AlienwildApricot.jpg)
Smell of fruit like apricots and perfumey
carbonated bite on front of tongue
Little to no hops
slightly bitter with fruit present through out reminescent of apricot kind of...
long finish difficult to really nail a description but a good thing.
Title: Re: Growing up an alien wild yeast from scratch
Post by: tschmidlin on February 22, 2011, 06:33:00 AM
I took a series of pics, but they all look more or less the same.  The morphology is very clearly yeast, with no rod-shaped bacteria evident in any of the samples I observed.

The pic below is from a 10-fold dilution of the sample 1vertical sent me.  There are some cells that are clearly budding, many are not, but the mothers and daughters may have been separated by vortexing when I mixed the sample.  That large clump in the middle is just detritus of some sort, there are smaller bits visible as well.

(https://lh3.googleusercontent.com/_j-Iuc3I_JMk/TWNUjVfG9yI/AAAAAAAAAHc/ikRwRrwk21E/s640/DSCN0014.JPG)

My apologies for taking so long to get a pic of this yeast, I'm mostly working in a computer lab now wrapping up my PhD, and I don't get over to my yeast lab much anymore.
Title: Re: Growing up an alien wild yeast from scratch
Post by: 1vertical on February 22, 2011, 02:30:19 PM
I have a few beers from this yeast left in that keg not many...then I gotta decide
to brew with it again or shine it on...(leaning toward shine it on)

Edit: Thanks so much for your contribution Tom