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Yeast and Fermentation / Banking Yeast
« on: November 17, 2016, 01:00:57 AM »
Has anyone had experience with propagating and banking yeast cultures for future use? I would like to have an alternative to always buying a pack of yeast for each brew day. I generally use the same yeast strains for my beers.
I have read a few articles on doing this. I have extensive experience in banking bacteria, but do not know if yeast behave differently when grown in culture, aliquoted into several sterile conical tubes, and then stored in a home freezer for several months (frost free).
I came across this article on HomeBrew Talk. http://www.homebrewtalk.com/freezing-yeast.html
I was used to using 50% glycerin final concentration, but this article states that 10% works better.
Any suggestions? Any experience with doing this?
I have read a few articles on doing this. I have extensive experience in banking bacteria, but do not know if yeast behave differently when grown in culture, aliquoted into several sterile conical tubes, and then stored in a home freezer for several months (frost free).
I came across this article on HomeBrew Talk. http://www.homebrewtalk.com/freezing-yeast.html
I was used to using 50% glycerin final concentration, but this article states that 10% works better.
Any suggestions? Any experience with doing this?