She wasn't extremely clear on the final dimensions she expected it to have. But it was to be field-size, using a bunch of fluidics and flow cell design that's way beyond me.
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I would trust microscope with hemocytometer rather than specs or plates.Plate counts would be the most accurate, since they'd give a viable count. However, you have to have decent technique (change pipettes/tips at each point or your numbers end up completely screwed up, and good ways to accurately measure volumes) and plenty of plates. Easy enough for me to manage in my lab with lots of micropipettes and access to an autoclave, but I'd hate to attempt it in the tiny kitchen in my apartment. In an ideal world you do growth curves correlating the optical density (probably at 600nm) with the plate counts in a growing sample -- again, this requires lots and lots of plates and seems like a hassle for someone like me who lacks dedicated space and equipment to do work with food organisms.
Acetobacter is aerobic - it often isn't much of a problem if you're kegging/bottle conditioning because there is no oxygen. But if you're cask conditioning and using ambient air to fill the headspace in the cask, then there will be plenty of oxygen in there (and you're probably innoculating with acetobacter too).Lactobacilli might be a problem, though, if they get in.
Pretty sure. Example thread. But there are probably differences in temperature vs the do-it-all-in-a-one-temp-apartment method I'm stuck with.I think part of my problem is that I keep reading about people getting carbonation in 3-5 daysAre you sure those people aren't talking about force carbonating? I don't naturally carbonate anymore, but I never got anything carbed that fast. It was always 2-3 weeks.