Just wanted to followup on this discussion. One week after harvesting I planned to pitch the whole 125 ml into a new 5.5 gal batch. I got a little nervous about the viability and pitched it into a 2 L starter. Then decanted and pitched as planned in 7 days. Fermentation took of nicely and collected another 140 ml from the second beer. What a sweet easy way of yeast collection, saving a little money, and having a little more fun.