I did a 48 sour mash on a partial boil recipe. The pH dropped to 3.5, then I did the boil for 15 and hopped it. I pitched a fresh vial of WLP001 and nothing. I figured 3 gal at 1.031 would be sufficient for a direct pitch. After 24 hrs nothing, so I added a cup of WLP001 slurry from a previous batch that had been in the fridge for a bit. Another 24hrs and nothing. I added some DAP nutirient incase the lacto didnt leave anything. After another 24 hrs, still nothing. So in a last gasp, I "broke the glass" and added my emergencrgy sachet of S05. Finally after 96hrs from first pitch I have active fermentation, but no real krausen to speak of.
OG was 1.031. Lacto took it to 1.022. SG is now down to 1.012 and still going. This thing scared me. My two cents, pitch a lot of active yeast at high krausen and feed with plenty nutrient. The harsh acid environment is not where you want to wake up a bunch of sleepy Sacch...