Morticaixavier, that is interesting. I was reading on Wyeast packaging, it mentions it contains 125 mls of yeast an nutrient. So, Brewer's Friend Pitch Rate Calculator predicts that if the viability is 1 billion per ml then a 1 liter starter would be perfect. But, if I intentionally underestimated it by 50% calling the viability .5 and using a starter of 2.7 to collect adequate cells. Do you think I would be over pitching in 5.5 gallons if I were wrong? Just trying to be on the safe side of viability.