Was making a 2 step starter for an IPA brew this weekend, using a vial of 001 with about 50% viability.
My plan was to make a 2L starter, stir that out and then dump that into my 5l flask with 3L of fresh wort. This should have given me 563bn cells vs. my required 482bn for this batch.
well, the 2L starter erupted on the stir plate, and it appears, based on the smell, look and consistency of the pooled liquid and streaks on the flask that I lost a significant amount of yeast down the sides of the flask. The liquid level in the flask settled at ~1850mL
I went ahead and pitched the step 1 into step 2, but now I'm wondering if I'll be anywhere close to my planned pitch rate.
Should I just scrap the whole thing and use US-05 this weekend, should i supplement the starter with a packet of US-05 when I pitch into the main wort, or should I just not worry about it?