So I am working on stepping up my WLP820 for My Märzen this weekend. I started with 1 vial (BB Date 10/23/14) and 1.8L wort in a 2L flask. Once chilled, I decanted most of the spent wort. The next step is 2.5L and was supposed to go into a 1Gallon wine bottle. Therein lies the problem. Once the 2.5L of fresh wort was in there and the slurry from original was added, It was just too much to be contained in the gallon bottle. I did use fermcap in both of the steps. The step sizes came from yeastcalc.com, supposed to yield 421B cell of my needed 423B cells. (this is all being done without a stir plate, only intermittent shaking) So here come the questions:
1. Upon realizing that I did not have enough room, I swirled the gallon container and then poured off 1.2L back into the erhlenmeyer flask. There were no visible solids upon this transfer so I feel that the yeast was well suspended. Will this arbitrary splitting yield 2 viable starters that can both be added to my wort on Saturday?
2. I have more DME if the consensus is that I need more volume, and will likely delay pitching till Sunday, so time is not a deterrant for any suggestions out there
So what say you all? Your thoughts and insights are greatly appreciated on this my first lager attempt. And a stir plate is in the works for future batches. Thank you in advance