As others have noted, refractometers are temperature sensitive. But wait you cry, my refractometer has ATC (auto temp correction). Well, yes and no. ATC works because the thermal mass of the big block of aluminum and quartz glass at the end of the refractometer, which has been sitting at room temperature, is significantly greater than the single drop of wort you are expected to add to it.
- If you pour a spoonful of boiling wort over the end, it will be too hot and read incorrectly.
- If you leave the refractometer in a hot humid brewery, it will be too hot and read incorrectly.
- If you keep it in your pocket, it will be too hot and read incorrectly.
- If you sit it on your brewstand 6" from the burner, it will be too hot and read incorrectly.
- Conversely, if your brewery or garage is icy cold, it will be too low and read incorrectly.
Bottom line, room temperature refractometer + 1 drop of wort as instructed and no air bubbles in the film = correct reading.