I'm making a starter for an IPA, and by popular request I'm doing a methylene blue stain and cell count on it.
Vital stats:
2 vials of WLP 001, expiration date Oct . 5 (produced on June 5)
2.5 L of canned starter wort:
- Best Pilz malt, enough calcium sulfate to hit 50 ppm ca, phosphoric acid to mash pH 5.4 room temp, yeast nutrient.
- No sparge, canned wort at 15 psi for 15 minutes.
- OG 1.045 or so. Higher than usual but part of an experiment with accommodating yeast to fermentation wort gravities.
I shook the starter for a whole minute, which is an eternity for one this big.
Put on the stir plate this morning and left it alone. After 10 hours:
I plan to stain and put on the microscope later, although if the krausen hasn't gone down yet before bed the cell count won't be accurate.