Author Topic: Orbital Shaker  (Read 2756 times)

S. cerevisiae

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Orbital Shaker
« on: June 27, 2015, 01:14:55 PM »
Well, I broke down and purchased a used Lab Line 3520 orbital shaker today.  If the shaker is in halfway decent operational shape, I will be happy because these things go for stupid amounts money on used lab equipment sites.  The unit is in good cosmetic shape for its age.  A review will follow after I receive the unit.






Offline tonyp

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Re: Orbital Shaker
« Reply #1 on: June 27, 2015, 02:55:34 PM »
Very cool gadget, if you're able can you take a few seconds of video with a flask? I would love to see it in action!
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Offline narcout

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Re: Orbital Shaker
« Reply #2 on: June 27, 2015, 03:26:35 PM »
Do you need a baffled flask to get the full effect?

I'd love to see a comparison (both cell counts and actual fermentation performance) among starters made on a stir plate, an orbital shaker, and shaken by hand across a variety of yeast strains, but I guess that would be a serious labor of love for the experimenter.

Very cool gadget, if you're able can you take a few seconds of video with a flask? I would love to see it in action!

Here is a really awesome YouTube video: https://www.youtube.com/watch?v=oMWYV49ME3o
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S. cerevisiae

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Re: Orbital Shaker
« Reply #3 on: June 27, 2015, 06:18:53 PM »
I am mostly interested in performance between a well-shaken by hand starter and starter shaken on an orbital shaker. 

The orbital shaker used at White Labs makes the 3520 look like a toy.  I believe that I saw six 5L flasks on the shaker at the same time.

I plan to use my 2L and 5L media bottles on the shaker.   A Fernbach flask (a.k.a. a squat baffled Erlenmeyer flask) can lead to excessive foaming.  I personally do not fear the foam, but over foaming in a conical flask can be a problem.


I need to purchase a new cover glass for my hemocytometer.  I have been putting the purchase off because a new cover glass for a Reichert Bright-Line hemocytometer costs more than a new Chinese hemocytometer.

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Re: Orbital Shaker
« Reply #4 on: June 27, 2015, 07:31:32 PM »
The orbital shaker used at White Labs makes the 3520 look like a toy.  I believe that I saw six 5L flasks on the shaker at the same time.

Did you see the big one in back? 3-4 racks of 8-10 flasks apiece if memory serves.
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Re: Orbital Shaker
« Reply #5 on: June 27, 2015, 09:31:06 PM »


a new cover glass for a Reichert Bright-Line hemocytometer costs more than a new Chinese hemocytometer.



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S. cerevisiae

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Re: Orbital Shaker
« Reply #6 on: June 27, 2015, 11:04:18 PM »

a new cover glass for a Reichert Bright-Line hemocytometer costs more than a new Chinese hemocytometer.



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A hemocytometer is used to perform yeast cell counts.  The device is called a hemacytometer because it was originally designed to count blood cells.


S. cerevisiae

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Re: Orbital Shaker
« Reply #7 on: June 27, 2015, 11:07:25 PM »
Did you see the big one in back? 3-4 racks of 8-10 flasks apiece if memory serves.

I was only able to get a quick glance of the room.

Offline klickitat jim

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Re: Orbital Shaker
« Reply #8 on: June 28, 2015, 05:59:29 AM »
Why couldnt an old turn table work with the flask just slightly off center?

Offline brewinhard

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Re: Orbital Shaker
« Reply #9 on: June 28, 2015, 07:59:14 AM »
Man, I bet you'll be able to whip up some killer martinis!  Bond style!

Offline klickitat jim

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Re: Orbital Shaker
« Reply #10 on: June 28, 2015, 09:37:22 AM »
On 78 for sure. 33 1/3 probably not too bad.

S. cerevisiae

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Re: Orbital Shaker
« Reply #11 on: June 29, 2015, 07:32:07 AM »
Why couldnt an old turn table work with the flask just slightly off center?

 I do not see why a turntable could not be pressed into duty if the orbit it not too large. 

With that said, the orbit can be adjusted on some modern shakers.  However, affordable and modern are mutually exclusive when talking about orbital shakers.  It appears that $600.00 is the starting point for a new orbital shaker with modern features.  Anything below this figure that is not pre-owned is junk.  That's a lot of money for a device that is a luxury, not a need.

Offline klickitat jim

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Re: Orbital Shaker
« Reply #12 on: June 29, 2015, 08:17:47 AM »
Right, cheap cheap at a garage sale and adjustable by where you put it on the turn table. I'll try to keep my eyes open for one.

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Re: Orbital Shaker
« Reply #13 on: June 29, 2015, 08:47:56 AM »
Right, cheap cheap at a garage sale and adjustable by where you put it on the turn table. I'll try to keep my eyes open for one.

That's a cool "out of the box" idea.  I look forward to seeing what you build.   I had a Pioneer PL-1000 turntable that I gave away back in the late nineties.  That turntable would have made a good platform for experimentation because the rotational speed was electronically controlled.

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Re: Orbital Shaker
« Reply #14 on: June 29, 2015, 09:30:25 AM »
If the shaker continually aerates the starter, will you still pitch the entire thing rather than chilling and decanting the supernatant?
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