Clean, sanitize with starsan, lube your o-rings and charge with 10psi of co2, just be sure to purge it a couple times before removing the gas line. If you have a fridge to put them in, do that because the cooler air will keep the pressure stable. When your ready to use again, just release the pressure and fill. I've done this many times over the years and have yet to need to replace any seals. As long as you sanitize it properly, have only co2 in the vessel, you will have no bacteria inside. Just be sure to spray the connections down with starsan before use as well.