so if you ferment under pressure you should not reuse the yeast?
I'm reusing it, as I said just above. Harvesting and repitching is important to me.
There's been some suggestion out there that pressure fermentation may cause long term harm to the yeast. But I think that may be specific to conditions like really high pressure (like 28 psig,) certain strains, or other things (like rapid decompression.)
If yeast couldn't be harvested and repitched, this would never have become a thing in German commercial practice. I think it just has to be handled correctly.
My 2nd generation is performing exactly like the first so far. I'm going to keep repitching it until it doesn't seem normal, or I get tired of making lagers, to see if it really does shorten the number of generations you can go. Stay tuned.
Some things I've taken into consideration in designing my process are, first, that pressure suppresses yeast growth, which could lead to less yeast in the current batch and fewer new cells in successive generations. To address that, my procedure is to pitch warm and leave it at atmospheric pressure until active, then let pressure build to 15 psig over a number of hours. This (based on the German process as described in Kunze, 4.4.3.5) should maximize growth. I've also targeted a pitch rate as for pitching at normal (cold) lager temperatures; this won't directly affect future generations, but will ensure sufficient yeast in the current batch. And to avoid sudden decompression that could rupture cells, I'm incrementally decreasing the pressure over a couple of days during cooling at the end of fermentation. If you don't want to reduce the pressure on the beer before transferring to a keg (say if you're spunding,) you could just slowly depressurize the fermenter after racking and before harvesting. Or not and let us know if it makes a difference.
All in all, I won't expect to take this yeast out the 18-25 generations some of us have previously done with lager yeasts. But I do expect to be able to get enough generations to make this a viable, practical method of lager fermentation. I'd be happy with 5.
Again, stay tuned. Can't wait for some others to get those Kegmenters and cornys and whatnot going and hear how your experiences compare.