I'm new to the liquid yeast world. So far, so good though. I'm just wondering if my method has any advantage over getting the slurry from a fermentor. I make an approximately 1800 ml starter and save around 300 ml from the starter and store it in a Mason jar. Next time I use the yeast I dump most of the Mason jar that's sitting on the settled cake and swirl it around to stir up the yeast and then dump it into my flask and off to the stir plate it goes. 5 gallon batches. My initial thought was that would be the purest version of the yeast, but am I overthinking it since it's new territory?
I think that's actually a great idea. Over build a starter, save some and rebuild that for the next brew. Definitely is going to be free from trub and dead yeast cells.
FWIW, I collect after cold crashing into a 1/2 gallon mason jar, tight lid (yea yea I know, leave the lid loose. never had an issue, yet
then I put it into my 36* refrigerator. I have read from various forums and magazines to use that yeast within two weeks. I have saved, then used harvested yeast that has been refrigerated for months and have had zero perceived issues. Obviously I build a nice vibrant starter and I tend to over pitch. I also never wash my harvested yeast. When building my starter with it I will try and leave whatever trub is on the bottom of the jar in place.