Oxidation is one of the most poorly understood aspects of brewing at the amateur and small to mid-size professional brewing level. Cardboard and Sherry-like flavors are the result of a problem upstream from fermentation (i.e., during malting and/or mashing). No amount of O
2 purging on the cold side will fix these off flavors. Given enough time, these flavors will show up. Beer that contains a live culture that is heavily oxidized during cold side transfers does not result in cardboard. It results in cidery and cider vinegar-like flavors due to the reduction of ethanol to acetaldehyde and then to acetate. In the presence of O
2 and no available carbon sources that can be reduced to a directly consumable hexose (i.e., sugars with the formula C
6H
12O
6), Crabtree positive yeast cells enter diauxic shift where they switch to using ethanol respiratively as their carbon source.
https://pathway.yeastgenome.org/YEAST/NEW-IMAGE?type=PATHWAY&object=PWY3O-4300“S. cerevisiae, as a Crabtree-positive yeast, predominantly ferments pyruvate to ethanol in high glucose conditions. When glucose or other preferred carbon sources are depleted, S. cerevisiae switches to aerobic respiration and utilizes ethanol as carbon source instead, a phenomenon known as diauxic shift. Ethanol degradation is a three-step pathway that begins with the oxidation of ethanol to acetaldehyde by alcohol dehydrogenase (ADH). Aldehyde dehydrogenase (ALD) then converts acetaldehyde to acetate, which acetyl-CoA synthetase (ACS) subsequently ligates with coenzyme A to produce acetyl-CoA.
Alcohol and aldehyde dehydrogenase each have several isozymes in S. cerevisiae. Of the alcohol dehydrogenases involved in catalyzing interconversion between ethanol and acetaldehyde, the cytosolic enzyme Adh2p is thought to preferentially catalyze ethanol oxidation to acetaldehyde due to its relatively low Km for ethanol. ADH2 is glucose-repressed and activated by Cat8p and Adr1p. The oxidation of acetaldehyde to acetate is catalyzed by both cytosolic and mitochondrial aldehyde dehydrogenase, of which the main isoforms (Ald6p and Ald4p, respectively) are important for growth on ethanol.”