Do you use a loop or needle to inoculate your stabs? With visible yeast on the one you use? Thanks for posting the log book.
to make a stab, let the agar set w/o resting the vial at an angle. Then take a sterilized needle and stick it into one of the colonies on the plate. After than just stab it a few times into the agar and let the culture grow a little before putting it into the frigde. I don’t think it matters much if you have visible yeast on the needle. I usually have a little bit.
Some are now as old as 3 years.
That means I have been able to keep the culture going for 3+ years. That involves periodical re-culturing. I stop harvesting the lawn once it starts looking brown. That usually happens after 4-7 months. At that point I may inoculate a new slant from the old one, get some yeast from a stab culture or start a plate culture from which I inoculate a new slant.
My oldest yeast is a culture of WY2206 and sometime in the future I want to brew a side-by-side with that yeast and a fresh yeast pack from Wyeast. I’m curious if I can detect taste and or fermentation performance differences.