#### javierdu

• 1st Kit
• Posts: 8
##### Question about multi step stater
« on: December 14, 2010, 12:19:31 AM »
starter question

Most of the information on the web, both written and calculators, talking of making a starter from yeast 100 billion for pack, unfortunately in Argentina, we are not able to get large packages, our suppliers bring to Wyest Propagator (about of 25 billion cells of yeast).

The question is how the steps to reach the required amount of for example 515 billion in yeast cells (counter example: Ale / 1075 / 10 gal) with only one pack of Propagator?

There a mathematical mind to let me handle these variables without requiring web calculators?

Javier

#### a10t2

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##### Re: Question about multi step stater
« Reply #1 on: December 14, 2010, 01:08:17 AM »
Is there a reason web calculators aren't an option for you?

The Wyeast calculator (http://www.wyeastlab.com/hb_pitchrate.cfm) will let you change from Activator to Propagator.

The MrMalty calculator (http://www.mrmalty.com/calc/calc.html) lets you set viability manually, just enter 25%.

It looks like you'll need about a 1.5 L first stage and 3 L second stage, using a stir plate or continuous aeration. Without a stir plate or air pump it would be three stages at 1 L, 2 L, then 5 L.
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#### javierdu

• 1st Kit
• Posts: 8
##### Re: Question about multi step stater
« Reply #2 on: December 14, 2010, 03:24:40 AM »
Thank you!

The simple reason is because I know from where the calculates (as unfortunately is not 1 +1 = 2).
I guess Wyest and Jamil are based on some previous calculations, which were wanted to know, Maybe, then Y¨ll pass to excel or graphics

javier

#### javierdu

• 1st Kit
• Posts: 8
##### Re: Question about multi step stater
« Reply #3 on: December 14, 2010, 10:16:34 PM »
can you explain how it can get 3 steps?

I think for the load you use mr malty
Step 1: 1022 / 10 gal / 25% = 158 billion / 4 pack / liter 1.1 req
Step 2: 1048 / 10 gal / 158% = 337 billion / 1 pack / 2 liter
Step 3: 1075 / 10 gal / 337% = 515 billion / 1 pack / 2 liters

I think I have something wrong because the third step does not give me 5 liters

Thank a lot