Denny and Mark, how are you guys cropping/ harvesting yeast?
Used to just pour the slurry from a fermenter into a sanitized container. Now I use the dump on my conicals.
Did you/ do you rinse the slurry, or just store it until reuse?
I tried rinsing it a long time ago and saw no advantage. Then Mark pointed that you can actually weaken the yeast by rinsing. Here's his quote from here on the forum...
Basically, it has always been a bad move that has been difficult to kill because every newbie wants to gain some street cred by publishing an article on yeast rinsing. Do you want to know where the practice of rinsing yeast with boiled water originated within the amateur brewing community? Charlie Papazian introduced it in the “New Complete Joy of Homebrewing” (it may even date back to an earlier publication of that book). He also promoted the use of a secondary fermentation vessel as a way to prevent autolysis. While using a secondary fermentation vessel to prevent autolysis has gone the way of the do-do bird, amateur brewers still cling to yeast rinsing, a practice that is not based on science and provides no microbiological advantage.
Brewing yeast strains are Crabtree postiive. What that means is that whenever the medium gravity is above the Crabtree threshold of 0.2% w/v (an S.G. of 1.0008), brewing yeast cultures will chose fermentation over respiration even in the prescence of O2. There is scientific evidence that brewing cultures became Crabtree positive due to competitive pressure. You see, the main reason why we have cell counts in the first place is that they are primarily a safeguard against a micro-oganism other than the pitched yeast culture owning the wort. From the time a yeast culture is pitched until the culture grows large enough to reach high krausen, it is in competition for ownership of the wort with wild microflora (boiled wort is not absolutely sterile and sanitization is not a synonym for sterilization). A yeast culture owns a batch of wort by doing three things. First, it consumes all of the dissolved O2, shutting out aerobic microflora. It then lowers the pH to around 4, which shuts out pH sensitive microflora. The pH sensitive microflora include the pathogen Clostridium botulinum, which cannot replicate below a pH of 4.6. The final defense that a yeast culture mounts is the production of ethanol, which is toxic to all living organism at a given level, even human beings (i.e., people die from alcohol poisoning every day).
When a brewer rinses yeast with boiled water, he/she removes the protective force field that a yeast culture built for itself, basically opening it up to infection from house microflora while providing zero microbiological advantage. A yeast culture does not need to be kept free from trub and hop particulate matter. It is needs to be kept as free from wild microflora as possible because every time a culture is pitched it is an opportunity for microflora other than the culture to replicate. This reality is what places an upper limit on bottom-cropped yeast more so that any other other reason when a yeast culture is not serially overpitched.
Now, top-cropping an interesting take on cropping. While the top-cropped yeast should also be stored under green beer, top-cropping naturally purifies a yeast culture because wild microflora do not floc to the top, which means that top-cropped yeast can be re-pitched almost indefinitely as long as care is taken to not infect the culture. The sad thing is that I have never heard of true top-cropping lager yeast.
If you need further evidence that yeast rinsing an amateur brewer fabrication that is not based on microbiology, watch how a craft or industrial brewery bottom crops yeast. They either pump it out of the cone into a yeast brink for temporary storage or into a fermentation vessel with fresh wort. I have yet to see a professional brewery rinse yeast with water before repitching it.