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Yeast Culturing

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Thirsty_Monk:
I just made my supply purchase at http://cynmar.com.


--- Code: ---12030895  GLASS ALCOHOL LAMP w/CAP, 4oz      US$3.40
CHA80228  DEHYDRATED AGAR POWDER, 30g      US$8.25
11527906  CLEAR GLASS VIALS w/AUTOCLAVABLE CAP, 12ml 12/PK      US$5.00
11527908 CLEAR GLASS VIALS w/AUTOCLAVABLE CAP, 16ml 12/PK US$5.75
13225101 NICHROME INOCULATING LOOP, 25 GAUGE WIRE, 8" TOT LENGTH US$1.50
13225110 NICHROME INOCULATING NEEDLE,25 GAUGE WIRE, 8"TOT LENGTH US$1.40
12030036 TEST TUBE BRUSH, WHITE NYLON, 3"BRUSH, 8" TOTAL US$0.70
13220697 GLASS PETRI DISH, 75x15mm,10/PK US$7.25

--- End code ---

Kai and his "Yeast culturing gear" http://braukaiser.com/wiki/index.php?title=Yeast_culturing_gear was very helpful.

I also have "First Steps In Yeast Culture"

read "Yeast Propagation and Maintenance: Principles and Practices" http://maltosefalcons.com/tech/yeast-propagation-and-maintenance-principles-and-practices
and listen yeast culturing episodes on Basic Brewing.

Anything else I should be aware of?
Thank you

hokerer:

--- Quote from: Thirsty_Monk on January 08, 2010, 12:35:51 PM ---Anything else I should be aware of?

--- End quote ---

30g of agar powder will last a good while but, once you do use that up, if you have a local Asian food store, you can usually get 25g of agar powder for about a buck twenty-five.

Kaiser:

--- Quote from: Geoff Henderson ---Anything else I should be aware of?
 
--- End quote ---

Keep a back up (dry yeast for example) on hand until you are confident.

Yes, agar from those lab places is expensive. I have yet to go to an Asian food store for that. I couldn’t get it at a health store.

Kai

a10t2:

--- Quote from: hokerer on January 08, 2010, 12:52:47 PM ---30g of agar powder will last a good while but, once you do use that up, if you have a local Asian food store, you can usually get 25g of agar powder for about a buck twenty-five.

--- End quote ---

Making your own from gelatin and DME works perfectly well for me, and I get some peace of mind from culturing the yeast on a maltose substrate.

mrdrysdale64:

--- Quote from: a10t2 on January 08, 2010, 02:18:11 PM ---
--- Quote from: hokerer on January 08, 2010, 12:52:47 PM ---30g of agar powder will last a good while but, once you do use that up, if you have a local Asian food store, you can usually get 25g of agar powder for about a buck twenty-five.

--- End quote ---

Making your own from gelatin and DME works perfectly well for me, and I get some peace of mind from culturing the yeast on a maltose substrate.

--- End quote ---

What proportions of gelatin and DME do you use? I assume you mix and then autoclave or put them in a pressure cooker? I need to try this. I have been kicking around the idea of culturing for a couple of years now. This forum is getting me motivated to do so many things!  :)

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