Author Topic: Yeast Culturing  (Read 6964 times)

Offline Thirsty_Monk

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Re: Yeast Culturing
« Reply #45 on: February 03, 2010, 07:51:17 PM »
It should be the same yeast so they should have similar properties.

Maybe they are not. One way of identifying yeasts for example is the shape of their giant colonies. Though the differences may be more pronounced between ale and lager there may even be differences between closely related strains.
Another reason to get microscop.

In the end it may not matter much how well they smear as long as they leave a trail of yeast cells.

I will be watching  ;D
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Online Kaiser

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Re: Yeast Culturing
« Reply #46 on: February 03, 2010, 08:08:11 PM »
Another reason to get microscop.


no microscope needed for that. I'm talking about the colony shape they form on a plate when they grow really large.

Kai

Offline dean

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Re: Yeast Culturing
« Reply #47 on: February 03, 2010, 08:10:01 PM »
I received my innoculation needle today... that sucker is HUGE!   :o  I was expecting something with a fine wire and point.  The whole thing must be 10 inches long and most of it is needle (closer in diameter to a coathanger really though!)  Well, I haven't been able to find any agar locally so I'm wondering if anybody has tried cornstarch... like making gravy but thicker so it stiffens more when its cooled.  Its generally clear and it seems corn is related closer to barley than grass maybe?  Anybody tried it?

I would try unflavored gelatin.  Corn starch will not solidify.

Oh yes it will... I've seen it gel up more than a few times.   :D

Offline Thirsty_Monk

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Re: Yeast Culturing
« Reply #48 on: February 03, 2010, 08:13:10 PM »
Another reason to get microscop.


no microscope needed for that. I'm talking about the colony shape they form on a plate when they grow really large.

Kai

I see.
Plates are week or so apart and I chilled later plate sooner so that might be a reason why colonies are smaller.
Na Zdravie

On Tap At The TapRoom:
Bohemian Pilsner
Bohemian Dark Lager
Smoked Bock
MaiBock
American Brown Ale
Marzen
Root beer

Offline Thirsty_Monk

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Re: Yeast Culturing
« Reply #49 on: February 04, 2010, 08:54:39 AM »
Both of yeasts are pretty happy in 10 ml wort.
Granted I shaked it about 2 hours ago.

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On Tap At The TapRoom:
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Bohemian Dark Lager
Smoked Bock
MaiBock
American Brown Ale
Marzen
Root beer

Online Kaiser

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Re: Yeast Culturing
« Reply #50 on: February 04, 2010, 09:00:35 AM »
I see.
Plates are week or so apart and I chilled later plate sooner so that might be a reason why colonies are smaller.

I innoculated a plate with the itent to grow a giant colony. Let's see how large it will get or if I get mold first.

Kai

Offline roffenburger

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Re: Yeast Culturing
« Reply #51 on: February 04, 2010, 10:11:28 AM »
I see.
Plates are week or so apart and I chilled later plate sooner so that might be a reason why colonies are smaller.

I innoculated a plate with the itent to grow a giant colony. Let's see how large it will get or if I get mold first.

Kai

What is the purpose of the giant colony? Just for fun?
Travis R.

Online Kaiser

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Re: Yeast Culturing
« Reply #52 on: February 04, 2010, 10:18:07 AM »
What is the purpose of the giant colony? Just for fun?

Yes, pretty much just for fun.

Kai

Online Kaiser

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Re: Yeast Culturing
« Reply #53 on: February 04, 2010, 10:41:24 AM »
here are some pictures of giant yeast colonies: http://prfdec.natur.cuni.cz/~zdenap/gallery.html

But they are not brewing yeasts and are also gown on stained agar.

Kai
« Last Edit: February 04, 2010, 10:52:37 AM by Kaiser »

Online Kaiser

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Re: Yeast Culturing
« Reply #54 on: February 04, 2010, 11:51:44 AM »
Here is some more info: http://brewingtechniques.com/library/backissues/issue2.3/morris.html

Maybe I should start an endavour to create a library of giant colony pics for a number of the yeast strains I culture.

Kai

Offline Thirsty_Monk

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Re: Yeast Culturing
« Reply #55 on: February 04, 2010, 12:09:12 PM »
here are some pictures of giant yeast colonies: http://prfdec.natur.cuni.cz/~zdenap/gallery.html
Those Czechs.
Who would think of growing giant yeast colonies :)
And by the way I can read the original version!!!

I will also read article from brewing techniques.
Thank you
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Bohemian Dark Lager
Smoked Bock
MaiBock
American Brown Ale
Marzen
Root beer

Online Kaiser

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Re: Yeast Culturing
« Reply #56 on: February 06, 2010, 04:05:35 PM »
If you are looking for purity, use the second method. This is streaking for isolation. Streak your first 1/3, sterilize (and cool)  your loop. Then make a couple passes back into the first third and into the second 1/3. sterilize and repeat.

I gave this a try the other day and besides making my plates look more prefessional it does seem to give me better colony isiolation.

Thanks,
Kai


Offline roffenburger

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Re: Yeast Culturing
« Reply #57 on: February 06, 2010, 05:50:20 PM »
If you are looking for purity, use the second method. This is streaking for isolation. Streak your first 1/3, sterilize (and cool)  your loop. Then make a couple passes back into the first third and into the second 1/3. sterilize and repeat.

I gave this a try the other day and besides making my plates look more prefessional it does seem to give me better colony isiolation.

Thanks,
Kai


hehe. Glad it worked out for you.
Travis R.

Online Kaiser

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Re: Yeast Culturing
« Reply #58 on: February 08, 2010, 10:17:32 PM »
hehe. Glad it worked out for you.

I sure am a convert. Look how nice it came out:



I'll have to take some time during the next days and update my Wiki with that technique.

Offline roffenburger

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Re: Yeast Culturing
« Reply #59 on: February 09, 2010, 09:35:35 AM »
I know I mentioned sterilizing the loop between quadrants, but its really not necessary. I mentioned it because most people not familiar with the techniques tend to take up an entire colony and streak it, which isn't needed. If you only take a very small amount, you don't have to flame your loop between quadrants to get isolated colonies.

Here are the two main streaks I do. I like to make a big third "quadrant" (I guess its not really a quadrant if I only do three sections...).


Travis R.