Author Topic: 3 month old Slurry - too old?  (Read 2281 times)

Offline bo

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Re: 3 month old Slurry - too old?
« Reply #15 on: September 01, 2011, 09:47:02 AM »
What's the longest amount of time anyone here has gone with freezing and had acceptable results?

Offline tschmidlin

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Re: 3 month old Slurry - too old?
« Reply #16 on: September 01, 2011, 09:53:49 AM »
You can freeze your yeast solid, but I think you are better off using enough glycerol to get it to freezer temps without freezing.  That way the cells are as cold as you can get them, but it avoids a lot of the problems that freezing causes.  For home, I think it is a good way to go.  But even in the fridge it can last a long time.  Rinse it a couple of times with distilled water and store it with distilled water and it should last you 6 months easily.
Tom Schmidlin

Offline bluesman

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Re: 3 month old Slurry - too old?
« Reply #17 on: September 01, 2011, 10:04:04 AM »
Rinse it a couple of times with distilled water and store it with distilled water and it should last you 6 months easily.

+1
Ron Price

Offline tschmidlin

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Re: 3 month old Slurry - too old?
« Reply #18 on: September 01, 2011, 10:05:34 AM »
What's the longest amount of time anyone here has gone with freezing and had acceptable results?
I've gone years with excellent results.  But mine are at -80C.
Tom Schmidlin

Offline bo

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Re: 3 month old Slurry - too old?
« Reply #19 on: September 01, 2011, 10:21:56 AM »
What's the longest amount of time anyone here has gone with freezing and had acceptable results?
I've gone years with excellent results.  But mine are at -80C.

Crap. My freezer only goes down to -70.

Offline tschmidlin

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Re: 3 month old Slurry - too old?
« Reply #20 on: September 01, 2011, 10:23:32 AM »
What's the longest amount of time anyone here has gone with freezing and had acceptable results?
I've gone years with excellent results.  But mine are at -80C.

Crap. My fridge only goes down to -70.
If you can really get them that cold then they will be fine for many years in a 20% glycerol solution.
Tom Schmidlin

Offline cenosillica

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Re: 3 month old Slurry - too old?
« Reply #21 on: September 01, 2011, 12:07:59 PM »
I think he was being sarcastic... -70 to -80 the yeast will be shivering so still that they won't notice the 10 degree difference.   :)


Great stuff! I'm going to try this glycerol mix.